在过去的几周里,我一直在尝试使用Scikit Allel库将基因组数据集加载到Neo4j中。我已经设法在VCF文件中加载外显子的所有变体以及所有具有相关表型数据的受试者,现在我正试图创建变体和受试者之间的关系。我对python不是很有经验,我认为这个问题不需要对基因组学或Scikit Allel库有很好的理解,所以不要被它吓跑
下面的代码可以工作,但速度非常慢。我认为为每个主题创建一个数据框架或列表集可能是一种更好的方法,而不是为j
for循环中的每个元素创建一个图形事务,但是对于如何最好地做到这一点,任何建议都将不胜感激。我也考虑过包括Numba,但不知道从哪里开始
这一过程每小时产生约1个新的受试者,每个染色体约1750个受试者和23个染色体,因此当前设置需要很长时间才能正常工作
import pandas as pd
import csv
import math
import allel
import zarr
from py2neo import Graph, Node, Relationship, NodeMatcher
zarr_path = '.../chroms.zarr'
callset = zarr.open_group(zarr_path, mode='r')
samples = callset[chrom]['samples']
graph = Graph(user="neo4j", password="password")
chrom_list = [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,'X']
for chrom in chrom_list:
variants = allel.VariantChunkedTable(callset[chrom]['variants'], names=['AC','AF_AFR', 'AF_AMR', 'AF_ASN', 'AF_EUR', 'AF_MAX', 'CGT', 'CLR', 'CSQ', 'DP', 'DP4', 'ESP_MAF', 'FILTER_LowQual', 'FILTER_MinHWE', 'FILTER_MinVQSLOD', 'FILTER_PASS', 'HWE', 'ICF', 'ID', 'IS', 'PC2', 'PCHI2', 'POS', 'PR', 'QCHI2', 'QUAL', 'REF', 'ALT', 'INDEL', 'SHAPEIT', 'SNP_ID', 'TYPE', 'UGT', 'VQSLOD', 'dbSNPmismatch', 'is_snp', 'numalt'], index='POS')
pos = variants['POS'][:]
SNPid = variants['ID'][:]
ref = variants['REF'][:]
alt = variants['ALT'][:]
dp = variants['DP'][:]
ac = variants['AC'][:]
vartype = variants['TYPE'][:]
qual = variants['QUAL'][:]
vq = variants['VQSLOD'][:]
numalt = variants['numalt'][:]
csq = variants['CSQ'][:]
vcfv = 'VCFv4.1'
refv = 'file:///lustre/scratch105/projects/g1k/ref/main_project/human_g1k_v37.fasta'
dpz = callset[chrom]['calldata/DP']
psz = callset[chrom]['calldata/PS']
plz = callset[chrom]['calldata/PL']
gpz = callset[chrom]['calldata/GP']
calldata = callset[chrom]['calldata']
gt = allel.GenotypeDaskArray(calldata['GT'])
hap = gt.to_haplotypes()
hap1 = hap[:, ::2]
hap2 = hap[:, 1::2]
i = 0
for i in range(len(samples)):
subject = samples[i]
subject_node = matcher.match("Subject", subject_id= subject)
if subject_node.first() is None:
continue
seq_tech = 'Illumina HiSeq 2000 (ILLUMINA)'
dp = dpz[:, i]
ps = psz[:, i]
pl = plz[:, i]
gp = gpz[:, i]
list_h1 = hap1[:, i].compute()
list_h2 = hap2[:, i].compute()
chrom_label = "Chromosome_" + str(chrom)
j = 0
for j in range(len(pos)):
h1 = int(list_h1[j])
h2 = int(list_h2[j])
read_depth = int(dp[j])
ps1 = int(ps[j])
PL0 = int(pl[j][0])
PL1 = int(pl[j][1])
PL2 = int(pl[j][2])
genotype = str(h1) + '|' + str(h2)
GP0 = float(gp[j][0])
GP1 = float(gp[j][1])
GP2 = float(gp[j][2])
k = int(pos[j])
l = str(ref[j])
m = str(alt[j][h1-1])
o = str(alt[j][h2-1])
if h1 == 0 and h2 == 0:
a1 = matcher.match(chrom_label, "Allele", pos= k, bp = l)
r2 = Relationship(subject_node.first(), "Homozygous", a1.first(), HTA=h1, HTB=h2, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r2)
elif h1 == 0 and h2 > 0:
a1 = matcher.match(chrom_label, "Allele", pos= k, bp = l)
r2 = Relationship(subject_node.first(), "Heterozygous", a1.first(), HTA=h1, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r2)
a2 = matcher.match(chrom_label, "Allele", pos= k, bp = o)
r3 = Relationship(subject_node.first(), "Heterozygous", a2.first(), HTB=h2, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r3)
elif h1 > 0 and h2 == 0:
a1 = matcher.match(chrom_label, "Allele", pos= k, bp = m)
r2 = Relationship(subject_node.first(), "Heterozygous", a1.first(), HTA=h1, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r2)
a2 = matcher.match(chrom_label, "Allele", pos= k, bp = l)
r3 = Relationship(subject_node.first(), "Heterozygous", a2.first(), HTB=h2, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r3)
elif h1 == h2 and h1 > 0:
a1 = matcher.match(chrom_label, "Allele", pos= k, bp = m)
r2 = Relationship(subject_node.first(), "Homozygous", a1.first(), HTA = h1, HTB = h2, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r2)
else:
a1 = matcher.match(chrom_label, "Allele", pos= k, bp = m)
r2 = Relationship(subject_node.first(), "Heterozygous", a1.first(), HTA=h1, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r2)
a2 = matcher.match(chrom_label, "Allele", pos= k, bp = o)
r3 = Relationship(subject_node.first(), "Heterozygous", a2.first(), HTB=h2, GT=genotype, seq_tech=seq_tech, dp=read_depth, phase_set=ps1, PL0=PL0, PL1=PL1, PL2=PL2, GP0=GP0, GP1=GP1, GP2=GP2)
graph.create(r3)
print("Subject " + subject + " completed.")
print(chrom_label + "completed.")
非常感谢您的帮助
单独创建大量元素总是很慢,这主要是因为需要的网络跳数。您还将在每一次之间进行匹配,这将进一步增加时间
解决这类问题的最佳方法是查看批处理,包括读取和写入。虽然您也不能同时完成所有操作,但一次将操作批处理成至少几百个操作将产生显著的效果。在您的情况下,您可能需要执行大容量读取,然后执行大容量写入,等等
因此,具体地说,可以针对多个实体进行匹配(您可以使用“in”修饰符,或者您可能需要使用原始密码)。对于写操作,使用相关节点和关系在本地构建一个子图,并在单个调用中创建该子图
您的最佳批量大小只能通过实验来发现,所以您可能不会第一次就得到正确的结果。但批处理无疑是这里的关键
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