亚基因组学工具包
mg-toolkit的Python项目详细描述
元基因组工具包使科学家能够下载所有的样本 给定研究或序列到单个csv文件的元数据。
安装元基因组工具包
pip install -U mg-toolkit
用法
$ mg-toolkit -h
usage: mg-toolkit [-h] [-V] [-d]
{original_metadata,sequence_search,bulk_download} ...
Metagenomics toolkit
--------------------
positional arguments:
{original_metadata,sequence_search,bulk_download}
original_metadata Download original metadata.
sequence_search Search non-redundant protein database using HMMER
bulk_download Download result files in bulks for an entire study.
optional arguments:
-h, --help show this help message and exit
-V, --version print version information
-d, --debug print debugging information
示例
下载元数据：
$ mg-toolkit original_metadata -a ERP001736
使用hmmer搜索非冗余蛋白质数据库并获取元数据：
$ mg-toolkit sequence_search -seq test.fasta -db full evalue -incE 0.02
Databases:
- full - Full length sequences (default)
- all - All sequences
- partial - Partial sequences
如何批量下载整个研究的结果文件？
$ mg-toolkit bulk_download -h
usage: mg-toolkit bulk_download [-h] -a ACCESSION [-o OUTPUT_PATH]
[-p {1.0,2.0,3.0,4.0,4.1}]
[-g {sequence_data,functional_analysis,taxonomic_analysis,taxonomic_analysis_ssu,taxonomic_analysis_lsu,stats,non_coding_rna}]
optional arguments:
-h, --help show this help message and exit
-a ACCESSION, --accession ACCESSION
Provide the study/project accession of your interest,
e.g. ERP001736, SRP000319. The study must be publicly
available in MGnify.
-o OUTPUT_PATH, --output_path OUTPUT_PATH
Location of the output directory, where the
downloadable files are written to. DEFAULT: CWD
-p {1.0,2.0,3.0,4.0,4.1}, --pipeline {1.0,2.0,3.0,4.0,4.1}
Specify the version of the pipeline you are interested
in. Lets say your study of interest has been analysed
with multiple version, but you are only interested in
a particular version then used this option to filter
down the results by the version you interested in.
DEFAULT: Downloads all versions
-g {sequence_data,functional_annotations,taxonomic_annotations,taxonomic_annot_ssu,taxonomic_annot_lsu,stats,non_coding_rna}, --result_group {sequence_data,functional_annotations,taxonomic_annotations,taxonomic_annot_ssu,taxonomic_annot_lsu,stats,non_coding_rna}
Provide a single result group if needed. Supported
result groups are: [sequence_data (all version),
functional_annotations (all version),
taxonomic_annotations (1.0-3.0), taxonomic_annot_ssu
(>=4.0), taxonomic_annot_lsu (>=4.0), stats,
non_coding_rna (>=4.0) DEFAULT: Downloads all result
groups if not provided. (default: None).
如何下载一个给定的学习加入的所有文件？
$ mg-toolkit -d bulk_download -a ERP009703
如何下载特定版本的研究结果？
$ mg-toolkit -d bulk_download -a ERP009703 -v 4.0
如何下载特定的结果文件组（例如，仅限功能注释）用于给定的研究登录？
$ mg-toolkit -d bulk_download -a ERP009703 -g functional_annotations
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