一个小的库,帮助Kseq用户选择引物。
primer-explorer2的Python项目详细描述
初级探索者2
一个小库,帮助K-Seq用户选择引物。在
作者:p.Ziarsolo,V.Garcia Carpino,J.Blanca
这个小的库提供了各种脚本来帮助用户为您的实验选择最好的kmers。在
底漆_xp.py版
It tries to select the best 10 primers for a K-Seq experiment and predicts the outcome of all 10 primer combinations.
The outcome consist in two file:
* An excel file with some stats for each primer combinations.
* A database with the predicted pcr products for each pair combination. This can be use as input for other provided scripts.
You can use the script to look for primers in different ways.
You can choose the length of the primer. The bigger is the length of the primer, less sites in the genome will find, thus generating less pcr products
You can define regions in witch you prefer to have less primers. P.ej: heterocromating, repetitive...
生成预测_床.py
^{pr2}$绘制产品尺寸_分布.py
It draws the insert size distribution of the predicted pcr products.
伯爵_功能.py
Given the prediction in bed files. It counts how many predicted pcr_products are in the gff features. By default in only counts pcr_produts in exons and genes. It can be changed.
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